Lee K. K , Cho J. J , Choi J. D , Jeongchon-ri , Seonggeo-eup , Cheonan-si , Chungnam

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Abstract

Inhibitory effects of 200 medicinal plants on elastase activity were screened. Among the 200 plants, six plant extracts exhibited more than 65% of inhibition on elastase activity by their total methanol extracts at 1, 000㎍/㎖ as a final concentration. For six active plants including Areca catechu(IC₅₀:42.4 ㎍/㎖), Cinnamonum cassia(IC₅₀:208.7 ㎍/㎖), Myristica frograns(lC₅₀:284.1 ㎍/㎖), Curcumma longa(lC₅₀:398.4 ㎍/㎖), Alponia katsumadai(lC₅₀:465.7 ㎍/㎖), and Dryopteris crassirrhizoma(IC₅₀:714.4 ㎍/㎖), the inhibitory effects of their comsituents were examined on the activity of human leukocyte elastase, hyaluronidase and lipid peroxidation. In lipid peroxidation assay using TBA method, three of the 6 plants including Curcumma longa(lC₅₀:45.5 ㎍/㎖), Areca Catechu(lC₅₀:51.0 ㎍/㎖) and Alponia katsumadai(lC₅₀:116.3 ㎍/㎖) exhibited more than 70% of inhibition at the concentration of 1, 000㎍/㎖, but only one plant, Areca Ctechu(lC₅₀:563 ㎍/㎖) showed high inhibitory effect on hyaluronidase activity. The results suggest that medicinal plants showing several biological activities may be potent inhibitors of anti-aging process on skin and that might be useful for application in cosmetics.

Key Words

Elasase, ANtioxidative activity, Hyakyribudase, Pkabt extracts, Inhibition, Elastase, Hyaluronidase, Plant extracts

이상화 Sanghwa Lee , 김진준 Jinjun Kim , 김호정 Hojeong Kim , 이종태 Jongtae Lee , 강세훈 Sehhoon Kang

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Melanin pigmentation in human skin is a major defense mechanism against ultraviolet light of the sun. Tyrosinase(EC 1.14.18.1) plays a key role in the biosynthesis of melanin. This is why much researches have been focused on its regulation in controlling the epidermal melanization. We have found that the water-extract of Banha(Pinelliae temate B.)，an oriental medicinal plant, has no tyrosinase inhibitory activity, but does inhibit the melanin biosynthesis in B16 mouse melanoma cells. We also found that Banha extract lowers the tyrosinase activity in cultured cells. To elucidate the action mechanism of Banha extract we have investigated its effect on the tyrosinase mRNA level using reverse transcription- polymerase chain reaction(RT-PCR) technique. It was revealed that Banha extract reduced the tyrosinase mRNA level in a dose dependent manner; when B16 mouse melanoma cells were cultured with 2mg/ml and 5mg/ml of Banha extract, there were 20% and 44% decrease in tyrosinase mRNA level, respectively. These data suggest that the Banha extract exerts its melanogenic inhibitoiy effect through the transcriptional regulation of tyrosinase mRNA.

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강현옥 Hyun-ok Kang , 정민석 Min-seok Juong , 최종완 Jong-oan Choi

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하병조 Byung-jo Ha , 김준오 Jun-oh Kim , 이옥섭 Ok-sub Lee

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Chitosan oligosaccharides having aldehyde group at reducing end were prepared by oxidative-deamination reaction of chitosan by using sodium nitrite, and the resulting aldehyde group was reduced to 2, 5-anhydro-D-mannitol group. The obtained chitosan oligosaccharides showed an average degree of polymerization 2~3 by gel permeation chromatography analysis. It was highly soluble in hydrophilic solvents and thermally stable. N, N-diacyl, O-acyl chitosan oligosaccharides were obtained from the reaction between chitosan oligosaccharides and acyl chloried under dimethylaminopyridine. From differential scanning calorimetric measurement, N, N-dilauroyl, O-lauroyl chitosan oligosaccharides showed mesophase region, which was confirmed by polarized microscope as a thermotropic liquid crystalline state. X-ray diffraction pattern revealed that N, N-dilauroyl, O-lauroyl chitosan oligosaccharedes were highly crystalline, whereas chitosan oligosaccharides were not.

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Ghang-tai Lee , Bong-soo Shin , Beom-jun Kim , Jeong-ha Kim , Byoung-kee Jo

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It has been observed that local increase in melanin synthesis or uneven distribution can cause local hyperpigmentation or spot. Pigmentary disorders are caused by various factors, including inflammation, imbalance of hormones, and genetic disorder[1]. Recently the harmfulness of Ultraviolet(UV) radiation is increasing due to destruction of ozone layer. Excessive exposure to UV radiation caused post-inflammatory pigmentation[2,3]. Most women want to avoid uneven skin pigmentation. To satisfy this desire many cosmetic companies have been developing melanogenesis inhibitors and finding promising active agents for use in cosmetic preparations for skin whitening. In cosmetic preparations, many inhibitors such as kojic acid[4], arbutin[5], ascorbic acid, and licorice extracts6 have been used as whitening purpose. Plant extracts having an inhibitory effect on melanin formation may be a good choice for cosmetic purpose because of their relatively lower side effects. Therefore, we screened 285 plant extracts for their inhibitory activity in tyrosinase[7]. Of the plant extracts, ramulus mori(the young twig of Morus alba L.) extracts showed potent tyrosinase inhibition activity. We also identified the active compound in the extract.

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조완구 W-g. Cho , B. P. Binks , P. D. L. Fletcher

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We have used a novel liquid surface forces apparatus(LSFA) to determine the variation of disjoining pressure with film thickness for dodecane-water-dodecane emulsion films. The LSFA allows measurement of film thicknesses in the range 5-100 nm and disjoining pressure from 0-1500 Pa. Disjoining pressure isotherms are given for films stabilised by the nonionic surfactants n-dodecyl pentaoxyethylene glycol ether(C₁₂E₅) and n-decyl-3-D-glucopyranoside(C₁₀-β-Glu) and the anionic surfactant sodium bis(2-ethylhexyl) sulphosuccinate(AOT) in the presence of added electrolyte. For C₁₂E₅ and AOT, the emulsion films are indefinitely stable even for the highest concentration of NaCl tested (136.7 mM) whereas the C₁₀-3-Glu film shows coalescence at this salt concentration. For film thicknesses greater than approximately 20 nm with all three surfactants, the disjoining pressure isotherms are reasonably well described in terms of electrostatic and van der Waals, forces. For the nonionic surfactant emulsion films, the charge properties of the monolayers are qualitatively similar to those seen for foam films. For AOT emulsion films, the monolayer surface potentials estimated by fitting the isotherms are similar to the values of the zeta potential measured for AOT stabilised emulsion droplets. For thin emulsion films(<20nm) certain systems showed isotherms which suggested the presence of an additional repulsive force with a range of approximately 20nm.

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이옥섭 , 이기화

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최정숙 Jeong Sook Choi

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Allergic contact dermatitis is a common skin disease resulting from specific immumologic sensitization to topically applied various allergen. Purpose of this study was to investigate skin morphologic chganges by light microscopic, changes of protein band by SDS-PAGE in the skin treated on the surfactant. Furthermore, lectin histochemistry is used to know the chagnges of the terminal sugar of the glycoconjugate in the skin treated on the surfactant. The results were as follows : The 1 day of treated group was indicated the enlargement of capillary, the 3 days of treated group was showed that the cytoplasm was eosinophlic by the pyknotic of nucleus. The 6 days of treated group was observed hyperkeratinoid, increased of inflammation cells to epithelium. The 9 days of treated group was appeares that serum crust was sheded and formed a acanthosis. But dermis layer was cytoplasmic vaculation and enlargement of intercellular space. The 12 days treated group made up new epidermis layer of seven layer and of observed an decreased in quantity of inflammation cells. The mast cell of degranulated type was increased in treated surfactnat. It was secreating granules hold histamin, serotonin and heparin. Accordingly, protein band of electrophortic phase was observed a sudden changes since 3 days treated group. At observeation of the cell-surface glycoconjugates, LCA, PNA, SBA and WGA lectin positive cells but inducated lectin negative cells in Con-A. Furthermore, PNA and SBA showed stroger positive reaction as treated surfactant group.

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